Isolate the best strains in hours

Synthetic Biology

Synthetic biology enables scientists to design new sequences of DNA from scratch to engineer novel biological functions and systems within microorganisms. Identifying genetically engineered microorganisms can be challenging due to their scarcity in the larger population. The small size of these microorganisms can make isolating them even more challenging.

Droplet-based RNA-seq (such as 10x Genomics’ Chromium system) can process more cells per assay than plate-based method, but only analyze 3’ UTR and capture about 1,000 genes per cell. Therefore, it cannot capture rare or low-expressing genes.

Plate-based RNA-seq can cover the whole transcription and capture more than 10,000 genes per cell. Traditional FACS single cell sorting method is harsh to the cells and affects cell integrity, resulting in gene expression changes and stress response. Namocell Single Cell Dispensers dramatically improve plate-based single cell RNA-seq.

Microorganisms are transfected with the desired genetic information along with a fluorescent reporter gene, such as GFP. Individual GFP-positive cells are then identified and dispensed with Namocell’s Single Cell Dispenser, then cultured for downstream characterization.

Namocell Benefits

  • Process 1 billion bacterial cells per hour

  • High cell recovery, no sample loss

  • Isolate microbes as small as 0.4 µm in diameter

  • No cross-contamination, no clogging
  • Easy to use, zero maintenance

Dispensing Efficiency

Namocell’s Single Cell Dispensers are highly efficient and consistent at dispensing single cells (80-90% singlets).

Namocell vs. FACS

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