The Pala Single Cell Dispenser is your benchtop analytical partner for single cell sorting. No matter what cell type you are working with, get single cells dispensed directly into your 96- or 384-well plates, all under 7 minutes!
By combining proprietary microfluidics, flow cytometry, and liquid dispensing technologies, this benchtop single cell sorter automates and simplifies single cell isolation across various applications – cell line development, cell engineering, single cell genomics, monoclonal antibody development, synthetic biology, and rare cell isolation such as circulating tumor cells (CTCs) and circulating fetal cells (CFCs).
The Pala Single Cell Dispenser is affordable, easy-to-use, zero maintenance, and requires no special training to operate!
Preparing and maintaining the instrument before and after the actual experiment—also known as overhead time—is the Achilles heel of many standard analytical instruments. The Pala Single Cell Dispenser drastically reduces overhead time, letting you go from initializing the instrument to achieving single cell isolation in as little as 7 minutes. This is in contrast to conventional FACS systems, which take over an hour to operate and provide results.
You can rest assured that the cells you get from the Pala system will be intact, healthy, and viable. The Pala system is gentle and uses very low pressure (<2 psi) for cell sorting, in contrast to high pressures (30-70 psi) typically used by conventional FACS sorters. The figure on the left shows a comparison of cellular stress response to pressures from FACS and Pala. Cells sorted with a FACS instrument showed induction of cellular stress response genes such as Ubiquitin C (Ubc), likely owing to high-pressure sorting conditions, while cells sorted on the Pala system showed much lower expression of these genes.
Pala is a compact, benchtop system which can easily fit into your tissue culture hood for sterile sorting. You can prepare your cells and easily isolate them in the same workstation!
Pala and other single cell sorters from Namocell utilize disposable microfluidic cell cartridges. Specifically designed for sterile isolation, each cartridge is a closed system, preventing cross contamination and aerosol formation.
The Pala system is automated and easy-to-use. It streamlines many of the steps required for traditional single cell sorters, therefore it doesn’t require any extensive training or specialized expertise. Anyone can run it!
| Excitation | Laser 1 – 488nm Laser 2 – 405nm or 561nm |
| Light Scatter | Forward (FSC) & Side (TSC) |
| Fluorescence | Up to 11 channels |
| Sorting Modes | Single Cell Dispensing Bulk Cell Sorting Target Cell Enrichment |
| Input Sample Volume | 100 – 600 uL |
| Input Sample Density | 100 cells – 150M cells/mL |
| Dispensing Volume | 1 uL/cell |
| Dispensing Format | 96- or 384-well plate, or microcentrifuge tube |
| Sorting Pressure | < 2 psi |
| Initialization Time | 5 min |
| Sheath Consumption | 6.5 mL/hr |
| Dimensions | 25 x 14 x 9 in 64 x 36 x 23 cm |
| Weight | 35 lbs 16kg |
| Compliance |  |
| Filter | 405nm | 488nm |
| 447/45 | DAPI, Pacific Blue, BFP | |
| 527/32 | Brilliant Violet 510, AmCyan | FITC, GFP, YFP |
| 586/42 | Brilliant Violet 570 | PE, DsRed, tdTomato, RFP |
| 625/15 | Brilliant Violet 605 | PE-Texas Red® |
| 692/80 | Brilliant Violet 650 | PerCP, PI |
| 783/56 | Brilliant Violet 785 | PE-Cy7 |
| Filter | 488nm | 561nm |
| 527/32 | FITC, GFP, YFP | |
| 586/42 | PE, DsRed, tdTomato, RFP | |
| 625/15 | PE-Texas Red®, mCherry | |
| 692/80 | PerCP, PI | PE-Cy5 |
| 783/56 | PE-Cy7 |
Namocell’s proprietary microfluidic cell cartridges
We’ve been using the Namo to do single cell sorting for genomics/ transcriptomics applications as well as for colony selection for tissue culture. We’re overall very satisfied with our results and find the setup to be very easy, as well as standard maintenance. After sterility protocols (which can take an hour), being able to immediately jump to sorting in less than ten minutes has saved us time and precious samples.
Noelani Kamelamela
Here at Cytovance, we love our Namocell. This machine has increased our plating capacity and efficiency nearly four-fold. The accuracy of Namocell’s 384-well plating coupled with the easy changes between samples has been a game changer for our workflow.
Mike Brem
Namocell Hana single cell dispenser provides a great solution for gentle single cell cloning using sterile disposable cartridges. The system is easy to use and quick to initialize. Hana combines the advantages of FACS and microfluidic cell dispensing to enable high assurance of monoclonality with high throughput and great cell viability.
Mandy Yim
Namocell’s disposable cartridges have allowed us to increase the throughput of the validation of our CRISPR engineered cell lines since we can sort a full plate in less than 3 minutes, and can move to the next plate without the hassle of cleaning. This outstanding product is paired with Namocell’s exceptional customer service that has been responsive and attentive to our needs. While evaluating different FACS machines, we simply did not find a rival that combined Namocell’s simplicity, performance and value, all while occupying a small footprint.
Trang Dickson
Our stem cell core facility was looking for ways to speed up our human induced pluripotent cell cloning to generate clonal cell lines and to analyze our gene editing efficiencies in single cells. Our criteria for an instrument for single cell dispensing was 1) sterility, 2) gentle on cells, 3) ease of use, and 4) reasonable cost. The Namocell fitted our criteria perfectly. We are able to reduce the time and effort for generating clonal CRISPR-engineered iPSC lines significantly. The instrument requires very little maintenance and users do not need to be highly trained with the main requirement being good aseptic technique. We are excited to use the Namocell potentially for other single cell applications.
Po-Lin So
