The Pala Single Cell Dispenser is your benchtop analytical partner for single cell sorting. No matter what cell type you are working with, get single cells dispensed directly into your 96- or 384-well plates, all under 7 minutes!
By combining proprietary microfluidics, flow cytometry, and liquid dispensing technologies, this benchtop single cell sorter automates and simplifies single cell isolation across various applications – cell line development, cell engineering, single cell genomics, monoclonal antibody development, synthetic biology, and rare cell isolation such as circulating tumor cells (CTCs) and circulating fetal cells (CFCs).
The Pala Single Cell Dispenser is affordable, easy-to-use, zero maintenance, and requires no special training to operate!
Preparing and maintaining the instrument before and after the actual experiment—also known as overhead time—is the Achilles heel of many standard analytical instruments. The Pala Single Cell Dispenser drastically reduces overhead time, letting you go from initializing the instrument to achieving single cell isolation in as little as 7 minutes. This is in contrast to conventional FACS systems, which take over an hour to operate and provide results.
You can rest assured that the cells you get from the Pala system will be intact, healthy, and viable. The Pala system is gentle and uses very low pressure (<2 psi) for cell sorting, in contrast to high pressures (30-70 psi) typically used by conventional FACS sorters. The figure on the left shows a comparison of cellular stress response to pressures from FACS and Pala. Cells sorted with a FACS instrument showed induction of cellular stress response genes such as Ubiquitin C (Ubc), likely owing to high-pressure sorting conditions, while cells sorted on the Pala system showed much lower expression of these genes.
Pala is a compact, benchtop system which can easily fit into your tissue culture hood for sterile sorting. You can prepare your cells and easily isolate them in the same workstation!
The Pala system is automated and easy-to-use. It streamlines many of the steps required for traditional single cell sorters, therefore it doesn’t require any extensive training or specialized expertise. Anyone can run it!
|Excitation||Laser 1 – 488nm|
Laser 2 – 405nm or 561nm
|Light Scatter||Forward (FSC) & Side (TSC)|
|Fluorescence||Up to 11 channels|
|Sorting Modes||Single Cell Dispensing|
Bulk Cell Sorting
Target Cell Enrichment
|Input Sample Volume||100 – 600 uL|
|Input Sample Density||100 cells – 150M cells/mL|
|Dispensing Volume||1 uL/cell|
|Dispensing Format||96- or 384-well plate, or microcentrifuge tube|
|Sorting Pressure||< 2 psi|
|Initialization Time||5 min|
|Sheath Consumption||6.5 mL/hr|
|Dimensions||25 x 14 x 9 in|
64 x 36 x 23 cm