Generating a clonal GFP cell line typically requires first sorting GFP-positive cells with a cell sorter. GFP-positive cells are then cultured for several days to recover from sorter induced cell stress. Clonal GFP cells are finally obtained by limiting dilution at one cell per well. In this application note, Namocell Single Cell Dispenser was used to directly isolate single GFP-positive cells into 96-well plate. Due to the low sorting pressure of the Namocell Single Cell Dispenser, clonal GFP cell lines can be generated in a single step.