Empower CRISPR Cell Engineering with Namocell

The Namocell Single Cell Dispensers are becoming a trusted partner that scientists rely on for single cell cloning to generate CRISPR-edited cell lines. From cell biology, to drug discover, to future cell therapy, discover the fascinating science that Namocell takes part in. 

Learn how Namocell Single Cell Dispensers are becoming a trusted partner that scientists rely on for single cell cloning to generate CRISPR-edited cell lines.

Fast, Gentle and Easy Single Cell Isolation for CRISPR & Cell Engineering

The CRISPR technology has enabled a new paradigm in which genes can be precisely modified, bringing immense potential into life science and biomedicine. In a typical CRISPR workflow, target cells are transfected with a plasmid containing both the CRISPR editing construct and a fluorescent reporter, enabling easy identification of successful transfections. Edited cells are then isolated for single cell cloning and clonal expansion. Single cell isolation has traditionally been done with two approaches, both of which have inherent drawbacks.

Limiting dilution has highly variable single cell isolation efficiency, often resulting in many empty wells or multiple cells per well making it inefficient and costly for clonal cell isolation. When compounded by low transfection or editing efficiency, it can be nearly impossible to isolate clones via limiting dilution. 

Fluorescence-Activated Cell Sorters (FACS), while faster and higher throughput than limiting dilutions, operate at high system pressure (up to 70 psi) that can easily damage or kill the sorted cells. Induced pluripotent stem (iPS) cells, a commonly used cell type for CRISPR cloning, are particularly sensitive to the high pressure and have low survival rates following FACS sorting. Additionally, conventional FACS is difficult to operate and prone to contamination.

Empower your CRISPR cell engineering research with high single cell dispensing efficiency and gentle single cell sorting with the Namocell single cell dispenser.

Take Your CRISPR Cell Engineering Research to the Next Level with Namocell Single Cell Dispensers

Fast Single Cell Isolation

Empower your cell engineering workflow with the fastest and simplest way to identify and isolate target single cells, all achieved seamlessly in one step.

High Single Cell Dispensing Efficiency

Cells are dispensed 1 cell/well at high efficiency into 96 or 384 well plates.

Gentle Sorting

Low-pressure sorting is gentle on your precious cells, preserving viability and integrity for better clonal outgrowth.

Embrace the Future of CRISPR and Cell Engineering

Single Cell Dispensing Efficiency

A comparative study was performed to serve as a reference for expected deposition efficiency. The results found that Namocell instruments are highly efficient and consistent at dispensing single cells (80-90% with mammalian cells) compared to manual pipetting (~30%).


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Discover how Namocell single cell dispensers are efficient and consistent at dispensing single cells (80-90% with mammalian cells) compared to manual pipetting.

Single Cell Cloning Efficiency

Namocell’s Single Cell Dispenser was used to sort CHO cells for clonal outgrowth alongside both limiting dilution (LDC) and a FACS. Cells sorted with Namocell showed higher cloning efficiencies than LDC and FACS.


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Namocell’s single cell sorter was used for isolate single cells for more efficient single cell cloning than LDC and FACS.
Dr. Oscar Perez-Leal, M.D, Assistant Professor, Temple University, School of Pharmacy utilized the Namocell single cell dispenser for single cell cloning in cell line development.
“Utilizing this machine (the Namocell Single Cell Dispenser) has proven instrumental in streamlining the development process of single-cell clones for the cell lines I am working on. It has greatly facilitated the generation of valuable research tools, contributing to the overall progress and efficiency of my work.”

Dr. Oscar Perez-Leal, M.D, Assistant Professor, Temple University, School of Pharmacy

“Our stem cell core facility was looking for ways to speed up our human induced pluripotent cell cloning to generate clonal cell lines and to analyze our gene editing efficiencies in single cells. Our criteria for an instrument for single cell dispensing was 1) sterility, 2) gentle on cells, 3) ease of use, and 4) reasonable cost….[With Namocell,] We are able to reduce the time and effort for generating clonal CRISPR-engineered iPSC lines significantly. The instrument requires very little maintenance and users do not need to be highly trained with the main requirement being good aseptic technique.”

Dr. Po-Lin So, Gladstone Institutes

Dr. Po-Lin So of Gladstone Institutes was able to minimize time and effort for generating clonal CRISPR iPSC cell lines with the Namocell single cell dispenser.

Featured CRISPR and Cell Engineering Resources

Dr. Oscar Perez-Leal of Temple University utilized Namocell single cell sorting to improve his workflow for CRISPR edited cell line development.

CRISPR-Edited Cell Line Development Workflow Improvements

Dr. Oscar Perez-Leal of Temple University explains how obtaining a pure clonal population of CRISPR-edited cells is critical yet challenging, and how the Namocell Single Cell Dispenser was instrumental in streamlining and accelerating the development of cell line models.

Access the From Your Peer Testimonial

Learn how Dr. Maks Prondzynski used Namocell single cell sorting to producing high quality CRISPR-Cas9 edited hiPSCs.

Webinar: Producing High Quality CRISPR-Cas9 Edited hiPSCs with Dr. Maks Prondzynski

Learn about optimizing the growth and differentiation of human induced pluripotent stem cells (hiPSCs) into cardiomyocytes to support enhancing cardiac disease modeling.

Watch Instantly On Demand

Learn how Namocell innovative single cell sorters were used to break barriers and advance research in CRISPR cell engineering.

Exclusive Coverage: Breaking Barriers in Cell Engineering with Single Cell Sorting Innovation

The team at Drug Target Review share insights, break down recent peer-reviewed publications, and talk one on one with scientists in this eBook focused on revealing how advances in single cell sorting technology are furthering research in CRISPR and cell engineering.

Download the Free eBook

Citations

High-throughput and automation advances for accelerating single-cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production. Tejwani V, et al. Biotechnol Prog. 2021 Nov;37(6):e3208. doi: 10.1002/btpr.3208. Epub 2021 Sep 20. PMID: 34478248.

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail. Tristan, C.A., et al. Nat Protoc 18, 58–80 (2023). https://doi.org/10.1038/s41596-022-00753-z

Integrating Micro and Nano Technologies for Cell Engineering and Analysis: Toward the Next Generation of Cell Therapy Workflows Prithvijit Mukherjee, et al. ACS Nano 2022 16 (10), 15653-15680. DOI: 10.1021/acsnano.2c05494

Age-Related Low Bone Mineral Density in C57BL/6 Mice Is Reflective of Aberrant Bone Morphogenetic Protein-2 Signaling Observed in Human Patients Diagnosed with Osteoporosis. Halloran D, et al. Int J Mol Sci. 2022 Sep 23;23(19):11205. doi: 10.3390/ijms231911205. PMID: 36232525; PMCID: PMC9570292.

Coexpression network and trans-activation analyses of maize reproductive phasiRNA loci. Zhan, J., et al. (2023), Plant J, 113: 160-173. https://doi.org/10.1111/tpj.16045

Identification of Inhibitors of Tubulin Polymerization Using a CRISPR-Edited Cell Line with Endogenous Fluorescent Tagging of β-Tubulin and Histone H1. Khachatryan H, et al. Biomolecules. 2023; 13(2):249. https://doi.org/10.3390/biom13020249

A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Chen, Y., et al. Nat Methods 18, 528–541 (2021). https://doi.org/10.1038/s41592-021-01126-2

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What Customers are Saying