Dr. Lina Chakrabarti from AstraZeneca highlights the benefits of using Namocell single cell dispensers in CHO cell line development. The instruments enhanced efficiency, predictability, and robustness of workflows, aiding safe and cost-effective biopharmaceutical manufacturing. The article details how Namocell instruments contributed to these improvements in cell line development.
The Chinese hamster ovary (CHO) cell line is widely used for the production of therapeutic antibodies. In a typical cell line development workflow, CHO cells are transfected with an immunoglobulin (IgG) expressing vector. After transfection, single, viable CHO cells are isolated for clonal expansion while high titer clones are screened for stability and productivity. These high producing CHO cell lines are then expanded for large scale antibody production.
Generating cell lines for therapeutic antibodies requires clonal cell populations. A critical first step involves isolating single, viable cells. Single cell isolation for cell line development has traditionally been done with either Fluorescence-Activated Cell Sorters (FACS) or limiting dilutions, each of which have inherent cost and efficiency drawbacks.
FACS instruments sort cells at high pressure (up to 70 psi), which is harsh on the cells and results in reduced cell viability. This manual screening method is also difficult to use and prone to cross-contamination and clogging.
Limiting dilution, while gentler than FACS, is time-consuming, labor-intensive, and offers poor single cell isolation efficiency. This has led to increased demand for automated, high-throughput solutions.
In CHO cell lines, CHO cells are transfected with a plasmid, encoding the desired protein for mass production. Following selection, transfected cells in suspension are loaded into one of Namocell’s proprietary microfluidic cell cartridges and dispensed with the Pala Single and Bulk Cell Sorter into 96- or 384-well plates for clonal outgrowth. These benchtop instruments offer quick, gentle cell isolation, helping to preserve cell viability and integrity. Following clonal expansion, colonies are analyzed via ELISA to determine antibody titers.
Empower your cell line development workflow with the fastest and simplest way to identify and isolate single cells, all achieved seamlessly in one single step.
Proprietary benchtop sorting technology is affordable, user-friendly, and requires zero special training. Fully automated with a low cost of operation.
Low-pressure sorting capabilities are gentle on your precious cells, helping to preserve viability and integrity throughout the cell line development workflow.
A comparative study was performed to serve as a reference for expected deposition efficiency. The results found that Namocell instruments are highly efficient and consistent at dispensing single cells (80-90% with mammalian cells) compared to manual pipetting (~30%).
Namocell’s Single Cell Dispenser was used to sort CHO cells for clonal outgrowth alongside both limiting dilution (LDC) and a FACS. Cells sorted with Namocell showed higher cloning efficiencies than LDC and FACS.
Dr. Oscar Perez-Leal, M.D, Assistant Professor, Temple University, School of Pharmacy
Dr. Lina Chakrabarti, Associate Director R&D, AstraZeneca
Results from Namocell single cell dispensers showed a 3x increase in IgG production from cold-captured cells compared to non-captured cells.
Dr. Oscar Perez-Leal of Temple University explains how obtaining a pure clonal population of CRISPR-edited cells is critical yet challenging, and how the Namocell Single Cell Dispenser was instrumental in streamlining and accelerating the development of cell line models.
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