Synthetic Biology

Synthetic biology enables scientists to design new sequences of DNA from scratch to engineer novel biological functions and systems within microorganisms. Identifying genetically engineered microorganisms can be challenging due to their scarcity in the larger population. The small size of these microorganisms can make isolating them even more challenging.

Microorganisms are transfected with the desired genetic information along with a fluorescent reporter gene, such as GFP. Individual GFP-positive cells are then identified and dispensed with Namocell’s Single Cell Dispenser, then cultured for downstream characterization.

Namocell Benefits

Fast

High throughput: process 3 million cells/min Work with sample density up to 150 million cells/mL

Efficient

High cell recovery, no sample loss

Precise

Isolate microbes as small as 0.4 µm in diameter

Compact

No cross-contamination, no clogging; Easy to use, zero maintenance

Dispensing Efficiency

Namocell Single Cell Dispensers are highly efficient and consistent at dispensing single cells (80-90% with mammalian cells) compared to manual pipetting (~30%).

Isolating Rare Cells

The Hana Single Cell Dispenser was used to isolate EpCAM+ cancer cells spiked into a background of white blood cells at a frequency of 1 in 10,000. Following single cell isolation, analysis of copy number variation across the genome confirmed successful isolation of the cancer cells (top panel), as compared with the background cells (bottom panel). As an internal control, the spiked-in cancer cells were female (XX) whereas the background cells were male (XY), as reflected in the results above.

What Scientists are Saying