Synthetic biology enables scientists to design new sequences of DNA from scratch to engineer novel biological functions and systems within microorganisms. Identifying genetically engineered microorganisms can be challenging due to their scarcity in the larger population. The small size of these microorganisms can make isolating them even more challenging.
Microorganisms are transfected with the desired genetic information along with a fluorescent reporter gene, such as GFP. Individual GFP-positive cells are then identified and dispensed with Namocell’s Single Cell Dispenser, then cultured for downstream characterization.
High throughput: process 3 million cells/min Work with sample density up to 150 million cells/mL
High cell recovery, no sample loss
Isolate microbes as small as 0.4 µm in diameter
No cross-contamination, no clogging; Easy to use, zero maintenance
Namocell Single Cell Dispensers are highly efficient and consistent at dispensing single cells (80-90% with mammalian cells) compared to manual pipetting (~30%).
Isolating Rare Cells
The Hana Single Cell Dispenser was used to isolate EpCAM+ cancer cells spiked into a background of white blood cells at a frequency of 1 in 10,000. Following single cell isolation, analysis of copy number variation across the genome confirmed successful isolation of the cancer cells (top panel), as compared with the background cells (bottom panel). As an internal control, the spiked-in cancer cells were female (XX) whereas the background cells were male (XY), as reflected in the results above.
What Scientists are Saying
Here at Cytovance, we love our Namocell. This machine has increased our plating capacity and efficiency nearly four-fold. The accuracy of Namocell’s 384-well plating coupled with the easy changes between samples has been a game changer for our workflow.
Namocell Hana single cell dispenser provides a great solution for gentle single cell cloning using sterile disposable cartridges. The system is easy to use and quick to initialize. Hana combines the advantages of FACS and microfluidic cell dispensing to enable high assurance of monoclonality with high throughput and great cell viability.
We’ve been using the Namo to do single cell sorting for genomics/ transcriptomics applications as well as for colony selection for tissue culture. We’re overall very satisfied with our results and find the setup to be very easy, as well as standard maintenance. After sterility protocols (which can take an hour), being able to immediately jump to sorting in less than ten minutes has saved us time and precious samples.
The Hana has had a positive impact on our workflow. We are now able, with a high degree of confidence, to generate clonal populations of cells with reduced cytotoxicity (compared to FACS) while simultaneously saving hands-on time due to not needing to immediately screen for single cells after manual limited dilutions. In many cell lines, we see a significantly higher efficiency of clonality from plate to plate compared to our previous methods.
The instrument works very well for our purposes of single-cell dispensing, primarily gene-edited lines. Easy to use; the setup and dispensing time is very short. It has the benefits of a FACS machine (binning by forward/side scatter), with lower pressure to help increase cell survival.
Namocell’s disposable cartridges have allowed us to increase the throughput of the validation of our CRISPR engineered cell lines since we can sort a full plate in less than 3 minutes, and can move to the next plate without the hassle of cleaning. This outstanding product is paired with Namocell’s exceptional customer service that has been responsive and attentive to our needs. While evaluating different FACS machines, we simply did not find a rival that combined Namocell’s simplicity, performance and value, all while occupying a small footprint.
Our stem cell core facility was looking for ways to speed up our human induced pluripotent cell cloning to generate clonal cell lines and to analyze our gene editing efficiencies in single cells. Our criteria for an instrument for single cell dispensing was 1) sterility, 2) gentle on cells, 3) ease of use, and 4) reasonable cost. The Namocell fitted our criteria perfectly. We are able to reduce the time and effort for generating clonal CRISPR-engineered iPSC lines significantly. The instrument requires very little maintenance and users do not need to be highly trained with the main requirement being good aseptic technique. We are excited to use the Namocell potentially for other single cell applications.