A powerful gene-editing technology, CRISPR allows for easy and quick ways to modify any genes in a given cell type. In a typical CRISPR workflow the cells are transfected with CRISPR constructs. A fluorescent-labeled marker can also be co-transfected. Once the cells have recovered, a single cell is isolated using limited dilution. The isolated single cells are allowed to grow. The knockout clones are identified with PCR. Limiting dilution is unreliable and many wells are either empty or have multiple cells. When high throughput CRISPR workflow is required, limited dilution becomes extremely inefficient and costly.