A powerful gene-editing technology, CRISPR allows for easy and quick ways to modify any genes in a given cell type. In a typical CRISPR workflow the cells are transfected with CRISPR constructs. A fluorescent-labeled marker can also be co-transfected. Once the cells have recovered, a single cell is isolated using limited dilution. The isolated single cells are allowed to grow. The knockout clones are identified with PCR. Limiting dilution is unreliable and many wells are either empty or have multiple cells. When high throughput CRISPR workflow is required, limited dilution becomes extremely inefficient and costly.

Namocell Single Cell Dispenser is able to dispense single cells into 90% of the wells. Using fluorescence sorting capabilities, Namocell Single Cell Dispenser is able to not only isolate single cells but also isolate knock-in or knockout cells when the gene is tagged with fluorescent proteins.

Application Note: